Helping people with computers... one answer at a time.
Without installing keystroke logging or other spyware on your computer, there's no way to tell if anyone is reading your emails before you do.
How do I know if someone is opening my email before I read it, then marking it as unread?
You don't. Essentially you would have to install some kind of keystroke logging or other spyware on your machine in order to see if someone is using it when you're not around.
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